论文部分内容阅读
毕赤酵母表达系统是一种高效的重组蛋白表达系统,近年来被广泛应用于重组蛋白的表达,它具有真核生物表达系统的特点,能够对目的蛋白进行类似高等真核生物的信号肽剪切、二硫键形成、糖基化等过程的翻译后蛋白加工,其宿主甲醇酵母—巴斯德毕赤酵母具有高密度生长的特性,本文从宿主菌、表达载体、表达载体的转化及整合和培养条件几个方面概述毕赤酵母表达系统的特点,以及该系统在寄生虫学领域中的应用进展。
Pichia pastoris expression system is a highly efficient recombinant protein expression system, which has been widely used in recent years, the expression of recombinant proteins, it has the characteristics of eukaryotic expression system, the target protein can be similar to higher eukaryotes signal peptide scissors Cut, disulfide bond formation, glycosylation and other processes of post-translational protein processing, the host methanol yeast Pichia pastoris with high-density growth characteristics, this paper from the host bacteria, expression vectors, expression vector transformation and integration And culture conditions in several aspects of the characteristics of the expression system of Pichia pastoris, and the application of the system in the field of parasitology.