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目的 研究人嗜T淋巴细胞病毒Ⅰ型相关性脊髓病/热带痉挛性截瘫(HAM/TSP)患者外周血淋巴细胞凋亡情况、HAM/TSP患者血清及脑脊液中sFas和sFasL表达水平及外周血Fas、肿瘤坏死引子α(TNF- α)和Bcl -2蛋白在HAM/TSP发生发展中的作用。方法 应用单细胞电泳检测HAM/TSP患者外周血淋巴细胞的凋亡情况,应用ELISA检测8例HAM/TSP患者血清和脑脊液中sFas、sFasL的水平,并用流式细胞术对患者外周血淋巴细胞上Fas、TNF -α和Bcl -2蛋白进行定量检测,并与健康对照组及其他神经系统疾病组(OIND)进行对比分析。结果 ( 1 )HAM/TSP患者外周血淋巴细胞凋亡率为13 .63%±0 .48%,与健康对照组(1 .63% ±0 .57% )比较显著增高(P<0 .05)。(2)患者血清sFas表达水平为(1 .08±0 .26)ng/ml,与OIND组[ (0 .29±0 .05)ng/ml]及健康对照组[ (0 .18±0 .07)ng/ml]比较显著增高(P<0 .05 );血清sFasL表达水平[ ( 0 .26±0 .11 )ng/ml]高于OIND组[ (0 .22±0 .04 )ng/ml]及健康对照组[ ( 0. 21±0 .05 )ng/ml],但差异无统计学意义(P>0 .05);脑脊液sFas和sFasL表达水平分别为( 0. 19±0 .07 )ng/ml和( 0. 24±0 .08 )ng/ml,显著高于OIND组[ (0 .11±0 .06)ng/ml、(0 .01±0 .01)ng/ml,P<0 .05]。(3)外周血淋巴细胞中Fas、TNF -α表达?
Objective To study the expression of sFas and sFasL in serum and cerebrospinal fluid (CSF) of patients with HAM / TSP and the level of peripheral blood Fas in patients with type I-associated myelopathy / tropical spastic paraplegia (HAM / TSP) , Tumor Necrosis Factor α (TNF-α) and Bcl -2 protein in the development of HAM / TSP. Methods The apoptosis of peripheral blood lymphocytes in HAM / TSP patients was detected by single cell electrophoresis. The levels of sFas and sFasL in serum and cerebrospinal fluid (CSF) of 8 patients with HAM / TSP were detected by ELISA. The peripheral blood lymphocytes Fas, TNF-αand Bcl-2 protein were detected and compared with the healthy control group and other neurological diseases group (OIND). Results (1) The apoptosis rate of peripheral blood lymphocytes in HAM / TSP patients was 13.63% ± 0.48%, which was significantly higher than that in healthy controls (1.63% ± 0.57%) (P <0.05) ). (2) The level of sFas in serum of patients was (1.08 ± 0.26) ng / ml, which was significantly higher than that of OIND group [(0.29 ± 0.05) ng / ml] and healthy control group [ (P0.26 ± 0.11) ng / ml] was significantly higher than that in OIND group [(0.22 ± 0.04), P <0.05] (0.21 ± 0.05) ng / ml], but the difference was not statistically significant (P> 0.05). The expression levels of sFas and sFasL in cerebrospinal fluid were (0 · 19 ± (0 .01 ± 0 .06) ng / ml, (0 .01 ± 0 .01) ng / ml and (0.24 ± 0.08) ng / ml were significantly higher than those in OIND group / ml, P <0 .05]. (3) The expression of Fas and TNF-α in peripheral blood lymphocytes?