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目的:探讨中等强度运动训练对心理应激大鼠行为和淋巴细胞热休克蛋白70表达的影响。方法:32只健康雄性SD大鼠随机分为4组:对照组、心理应激组、运动组和运动+心理应激组,每组8只。对照组不进行运动和心理应激干预,正常饲养;心理应激组前7周同对照组,第8周采用每天一次、每次2小时、持续3周的噪音刺激诱发建立心理应激模型;运动组每天无负重游泳训练1次,每次60分钟,共10周;运动+心理应激组运动10周,从第8周开始附加与心理应激组同样的应激。实验后测定各组大鼠旷场行为和外周血淋巴细胞热休克蛋白70表达。结果:(1)与对照组相比,心理应激组和运动组大鼠爬格块数均显著增加(P=0.024,P=0.047),心理应激组和运动组大鼠直立次数显著降低(P=0.03,P=0.001);双因素方差分析表明,运动训练和心理应激交互作用对大鼠爬格块数(F=6.30,P=0.02)和直立次数(F=6.91,P=0.01)有显著影响。(2)与对照组相比,心理应激组和运动+心理应激组大鼠热休克蛋白70表达均显著增加(P<0.01),运动组热休克蛋白70显著下降(P=0.04);双因素方差分析表明中等负荷运动训练和心理应激交互作用对大鼠淋巴细胞热休克蛋白70表达有显著影响(F=4.26,P=0.048)。结论:中等强度运动训练能减小心理应激导致的大鼠行为和淋巴细胞热休克蛋白70表达的变化,提示适宜强度的运动能够拮抗心理应激对机体造成的损伤。
Objective: To investigate the effect of moderate intensity exercise training on behavior and lymphocyte heat shock protein 70 expression in psychological stress rats. Methods: Thirty-two healthy male SD rats were randomly divided into 4 groups: control group, psychological stress group, exercise group and exercise + psychological stress group, 8 rats in each group. The control group did not exercise and psychological stress intervention, normal feeding; Psychological stress group 7 weeks before the control group, the first 8 weeks using a day, every 2 hours for 3 weeks of noise stimulation induced the establishment of psychological stress model; The exercise group had no weight-bearing swimming training once every day for 60 minutes for 10 weeks. The exercise + psychological stress group exercised for 10 weeks, and the same stress as the psychological stress group was added from the 8th week. After the experiment, the open field behavior and the expression of heat shock protein 70 in peripheral blood lymphocytes in each group were measured. Results: (1) Compared with the control group, the number of platyclampsia in both psychological stress group and exercise group increased significantly (P = 0.024, P = 0.047), while the number of standing in psychological stress group and exercise group decreased significantly (P = 0.03, P = 0.001). Two-way analysis of variance showed that the interaction between exercise training and psychological stress had no significant effect on the number of climbing blocks (F = 6.30, P = 0.02) and upright number (F = 6.91, P = 0.01) have a significant impact. (2) Compared with the control group, the expression of heat shock protein 70 in both psychological stress group and exercise + psychological stress group increased significantly (P <0.01), while that of exercise group decreased significantly (P = 0.04). Two-way analysis of variance showed that the interaction of moderate-load exercise training and psychological stress had a significant effect on the expression of heat shock protein 70 (F = 4.26, P = 0.048). CONCLUSION: Moderate-intensity exercise training can reduce the behavioral changes induced by psychological stress and the expression of heat shock protein 70 in lymphocytes, suggesting that exercise with appropriate intensity can antagonize the damage caused by psychological stress on the body.