1964～1971年间在美国加州对263000人进行了一次常规健康体检,所取血样于-40℃冰箱保存。1986年对其中151名在此阶段中发生肺癌的患者和320名对照的血清视黄醇水平进行了测定和比较。结果发现血清视黄醇与肺癌发生无关。之后作者又对血清中β-胡萝卜素含量进行了测定。用高效液相色谱法同时测定123例肺癌和246例对照血清中的视黄醇、β-胡萝卜素和α-生育酚的含量,结果发现病例组血清胡萝卜素水平(50.0±5.2μg/L)低于对照组(59.1±3.1μg/L),但无统计学显著性(P=0.13)。然而,如将血清β-胡萝卜素分成5级后进行logistio回归分析,则发现血清β-胡萝 Between 1964 and 1971, a routine physical examination of 263,000 people was conducted in California, USA. Blood samples were stored in a refrigerator at -40 ° C. In 1986, 151 serum levels of retinol in patients with lung cancer and 320 controls at this stage were measured and compared. The results showed that serum retinol and lung cancer has nothing to do. After that, the author determined the content of β-carotene in serum. Simultaneous determination of retinol, β-carotene and α-tocopherol in 123 lung cancer patients and 246 control subjects by high performance liquid chromatography showed that serum carotene levels (50.0 ± 5.2 μg / L) Lower than the control group (59.1 ± 3.1μg / L), but not statistically significant (P = 0.13). However, if serum beta-carotene is divided into 5 levels and logistio regression analysis is performed, it is found that serum beta-carrot