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The roles of different point defects in persistent luminescence of SrAl2O4:Eu,Dy phosphors were investigated. phors. It can serve as the electron trap of suitable depth for persistent luminescence. V(o) does not serve as the electron trap work as an effective electron trap. The point defect of V"Sr can be hole trap, but the change of its density in crystal matrix does not arouse the obvious change of persistent luminescence.