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[目的]观察A型肉毒毒素(BTX-A)对大鼠幽门离体平滑肌自发性收缩和P物质(SP)引发的幽门平滑肌收缩的影响,并初步探讨其机制。[方法]幽门离体平滑肌随机分为对照组、SP组、SP+BTX-A组、SP+NK1受体拮抗剂组,Biolap420E生物机能实验系统记录胃肌条收缩情况。[结果]①SP引发幽门平滑肌幅度增强(P﹤0.05),②BTX-A抑制SP引发的幽门平滑肌幅度(P﹤0.01),NK1受体拮抗剂抑制SP引发的幽门平滑肌张力(P﹤0.01)。[结论]SP增强幽门平滑肌收缩,BTX-A及NK1受体拮抗剂均抑制SP引发的幽门平滑肌收缩。考虑BTX-A可能通过抑制突触前膜递质释放作用,或抑制SP与突触后膜上相应受体结合发挥作用。
[Objective] To observe the effect of botulinum toxin type A (BTX-A) on spontaneous contractions of isolated pyloric smooth muscle and substance P (SP) -induced pyloric smooth muscle contraction in rats and its mechanism. [Method] The isolated smooth muscle of pylorus was randomly divided into control group, SP group, SP + BTX-A group, SP + NK1 receptor antagonist group and Biolap420E bio-functional experimental system. [Results] ① The amplitude of pyloric smooth muscle stimulated by SP increased (P <0.05). ② BTX-A inhibited the amplitude of pyloric smooth muscle induced by SP (P <0.01). NK1 receptor antagonist inhibited the pyloric smooth muscle tension induced by SP (P <0.01). [Conclusion] SP can enhance the contraction of pyloric smooth muscle. Both BTX-A and NK1 receptor antagonist inhibit the contraction of pyloric smooth muscle induced by SP. It is considered that BTX-A may play a role by inhibiting the presynaptic membrane neurotransmitter release or inhibiting the SP binding to the corresponding receptor on the postsynaptic membrane.