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目的探讨胎鼠生长受限(FGR)时大鼠胰岛素生长因子Ⅰ(IGF-Ⅰ)及其受体(IGF-ⅠR)表达的变化及其在组织和细胞中的定位。并探讨川芎嗪注射液对FGR的治疗作用。方法采用烟酒混合因素建立大鼠FGR模型,部分孕鼠予川芎嗪注射液8mg/kg治疗,3组均于孕20日剖宫取出胎鼠,比较3组胎鼠的体重、鼻臀长度、体重系数。并采用免疫组化法测孕晚期胎盘和胎鼠肝脏中IGF-Ⅰ和IGF-ⅠR的表达。结果实验组胎鼠体重、身长较对照组明显降低(P<0.05),实验组胎盘IGF-Ⅰ及胎鼠肝脏IGF-IR表达明显降低(P<0.05),而胎盘IGF-IR及胎鼠肝脏IGF-Ⅰ表达则明显提高(P<0.05),川芎嗪注射液治疗效果明显。结论IGF-Ⅰ及其受体水平与FGR发病相关,川芎嗪注射液可能通过直接(或间接)促进IGFs的合成和分泌,促进胎儿宫内发育。
Objective To investigate the expression of insulin-like growth factor I (IGF-I) and its receptor (IGF-IR) in fetal rat growth restriction (FGR) and its localization in tissues and cells. And discuss the Ligustrazine injection on the treatment of FGR. Methods Rat FGR model was established by using alcohol and alcohol mixed factors. Some pregnant rats were treated with Ligustrazine injection 8 mg/kg. All 3 groups were fetalized on cesarean section on the 20th day of pregnancy. The body weight, length of hips, and the length of the three groups were compared. Weight coefficient. Immunohistochemistry was used to measure the expression of IGF-I and IGF-IR in the placenta of the third trimester of pregnancy and the fetal liver. Results The body weight and length of the fetal rats in the experimental group were significantly lower than those in the control group (P<0.05). The expression of IGF-I in the placental IGF-I and fetal liver of the experimental group was significantly lower (P<0.05), while the placental IGF-IR and fetal rat liver were significantly decreased. The expression of IGF-I was significantly increased (P<0.05). Ligustrazine injection had a significant therapeutic effect. Conclusions The levels of IGF-I and its receptors are associated with the pathogenesis of FGR. Ligustrazine injection may promote the synthesis and secretion of IGFs directly and indirectly, and promote intrauterine growth.