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利用SSR检索程序从两个辣椒转录组共221037条unigenes(97.3Mb)中筛选得到17319个SSR位点(7.83%),其发生频率为1/56kb。其中以单核苷重复基序为主导类型,占总SSR的56.30%;其次是二、三核苷酸重复基序,其出现频率分别为19.16%和23.18%。对所有含SSR位点EST序列设计的10468对引物进行E-PCR多态性检测,获得1538对E-PCR多态性引物。随机选取20对引物进行验证,其中7对(36.84%)在10个不同类型辣椒材料中表现出多态性,PIC值范围为0.33～0.89,平均为0.67,将10个辣椒材料分为3类。这些潜在的多态性EST-SSR的开发为辣椒遗传多样性分析、图谱构建提供了更丰富的候选分子标记。
A total of 17319 SSR loci (7.83%) were screened from 221037 unigenes (97.3Mb) of two capsicum transcriptomes using the SSR search program and occurred at a frequency of 1 / 56kb. The dominant single nucleotide repeat motifs accounted for 56.30% of the total SSRs, followed by two or three nucleotide repeat motifs, with frequency of 19.16% and 23.18%, respectively. A total of 10,468 pairs of primers designed for the EST sequences containing SSR sites were tested for E-PCR polymorphism and 1538 pairs of E-PCR polymorphic primers were obtained. Twenty randomly selected primers were validated, of which 7 pairs (36.84%) showed polymorphism in 10 different types of pepper materials with PIC values ranging from 0.33 to 0.89 with an average of 0.67. Ten pepper materials were divided into three groups . The development of these potential polymorphic EST-SSRs provided a wealth of candidate molecular markers for genetic analysis of pepper genetic diversity.