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目的:对黄芪对大鼠失神经早期骨骼肌细胞凋亡以及凋亡相关蛋白活性的影响进行分析探讨,为今后的实验研究提供可靠的参考依据。方法:抽取SD大鼠69只,建立右下肢腓肠肌神经支配模型,随机分成3组,分别定义为失神经对照组、失神经黄芪干预组和阴性对照组,对术后2、14、28天时大鼠腓肠肌骨骼肌细胞凋亡细胞核以及凋亡的相关蛋白水平进行检测,并对检测结果进行对比分析。结果:失神经对照组、失神经黄芪干预组大鼠细胞凋亡较阴性对照组发生显著增加(P<0.05);术后14天和28天时黄芪干预组大鼠的腓肠肌湿重较同时期失神经组发生显示升高(P<0.05),然而凋亡细胞核、凋亡相关蛋白Caspase-8,Caspase-9活性则较失神经对照组发生显著降低(P<0.05)。结论:由以上研究可证实黄芪具有对大鼠失神经早期骨骼肌细胞凋亡产生显著的延缓作用,并且对Caspase-8,Caspase-9活性也能够产生一定的抑制效果,值得关注。
Objective: To study the effect of Astragalus membranaceus on the apoptosis of skeletal muscle cells and the activity of apoptosis-related proteins in the early stage of denervation in rats, and provide a reliable reference for the future experimental research. Methods: 69 SD rats were selected and the right lower extremity gastrocnemius innervation models were established. They were randomly divided into 3 groups, which were defined as the denervated control group, the denervated astragalus intervention group and the negative control group, respectively. After 2, 14 and 28 days, Apoptotic nuclei of the gastrocnemius muscle and the related protein levels of apoptosis in the gastrocnemius muscle were detected, and the test results were compared. Results: Compared with the negative control group, apoptosis of the rats in the denervated and denervated Astragalus membranaceus group was significantly increased (P <0.05); the wet gastrocnemius weight of the rats in the Astragalus membranaceus group was 14 and 28 days after the operation (P <0.05). However, the activity of Caspase-8 and Caspase-9 in apoptotic nuclei and apoptosis-related proteins were significantly lower than those in control group (P <0.05). Conclusion: Astragalus can delay the apoptosis of skeletal muscle cells in early stage of denervation in rats, and inhibit the activity of Caspase-8 and Caspase-9 to a certain extent, which deserves our attention.