论文部分内容阅读
目的:研究预电刺激小脑顶核对大鼠脑缺血再灌注后Ku70 mRNA的表达及其神经保护的分子机制。方法:Wistar大鼠通过原位杂交的方法及末端标记法检测Ku70 mRNA的表达及Tunel阳性细胞数。结果:①单纯造模组及毁损小脑顶核组缺血/再灌注后各时点Ku70 mRNA的表达无显著性差异,均较预刺激组及假手术组明显减少(P<0.01),预刺激组除缺血/再灌注后6h Ku70mRNAR的表达较假手术组减少(P<0.01)外,余时点与假手术组Ku70 mRNA表达无明显差异;②预刺激组Tunel阳性细胞数较未给预电刺激的两组明显减少(P<0.01)。结论:①预电刺激小脑顶核能减少缺血区神经元凋亡可能与DNA修复酶Ku70活性上调有关;②毁损小脑顶核后电刺激对脑缺血/再灌注引起的氧化性DNA损伤无保护作用。
OBJECTIVE: To study the expression of Ku70 mRNA and the molecular mechanism of neuroprotection after pre-stimulation of cerebellar fastigial nucleus on cerebral ischemia-reperfusion in rats. Methods: The expression of Ku70 mRNA and the number of Tunel positive cells in Wistar rats were detected by in situ hybridization and terminal labeling. Results: ①The expressions of Ku70 mRNA in the ischemic / reperfused cerebellar nuclei group were significantly lower than those in the pre-stimulation group and the sham-operated group (P <0.01) There was no significant difference in the expression of Ku70 mRNA between the time point and the sham-operated group except for the expression of Ku70mRNAR at 6h after ischemia / reperfusion (P <0.01). ②The number of Tunel positive cells in the pre-stimulation group was lower than that in the sham operation group The electrical stimulation of the two groups was significantly reduced (P <0.01). Conclusion: Pre-electrical stimulation of cerebellar apical degeneration of neurons in ischemic zone may be related to the upregulation of DNA repair enzyme Ku70 activity; ② electrical stimulation of cerebellar fastigial nucleus after cerebral ischemia / reperfusion-induced oxidative DNA damage without protection effect.