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The 10Ni5CrMoV steel examined was a 16 mm thick plate. Specimens measuring 12 mm × 12 mm × 120 mm were thermally cycled using DM-100 A weld simulator with various parameters. The main results are summarized as follows. In the coarse-grained austenitized region( Tm = 1 300℃ + 1 300℃ ) ,the microstructure is in good toughness. At the condition of Tm = 1 300℃ + 850℃ and ts/5 = 43 s, the toughness decreases heavily because M-A constituent and twin martensite appear at the prior austenite grain boundaries. When Tm = 1 300℃ + 850℃ or 1 300℃ + 730℃ and t/v5 = 85 s, local brittle zone is formed because of relatively coarse granular bainite.