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探讨猪肝细胞膜蛋白结构与异种移植细胞免疫反应原性的关系。方法:肝衰大鼠分为冻存细胞移植组(17只)、新鲜细胞移植组(9只)和对照组(9只,不移植肝细胞),腹腔内分别移植新鲜和冻存的猪肝细胞,记录鼠的存活率。圆二色光谱测定新鲜和冻存猪肝细胞的膜蛋白构象,抗体依赖性细胞毒性(antibody-dependentcellular cytotoxicity, ADCC)试验检测肝细胞破损情况。结果:对照鼠存活 3只(3/9),新鲜细胞移植鼠存活 6只(6/9);移植冻存肝细胞后,存活 16只(16/17),显著高于对照鼠,P=0.002 2。新鲜肝细胞膜蛋白构象中α螺旋比率为47.5%,经液氮处理后,α螺旋增至56.5%;ADCC显示存活的新鲜细胞数为0.24 × 105,冻存细胞0.68 × 105。结论:异种移植细胞免疫反应原性的降低与肝细胞膜蛋白中α螺旋比率增高有关。
To investigate the relationship between porcine hepatocyte membrane protein structure and immunogenicity of xenografted cells. Methods: The rats with liver failure were divided into three groups: the frozen-cell transplantation group (17), the fresh-cell transplantation group (9) and the control group (9 without transplantation of liver cells) Cells, recording the survival rate of the mice. Circular dichroism spectroscopy was used to detect the membrane protein conformation and the antibody-dependent cellular cytotoxicity (ADCC) assay of fresh and frozen porcine hepatocytes. RESULTS: The control mice survived 3 (3/9) and the fresh cells transplanted mice survived 6 (6/9). After transplanted frozen hepatocytes, 16 (16/17) survived, which was significantly higher than that of the control mice (P = 0.002 2. The α-helix ratio of fresh hepatocyte membrane protein conformation was 47.5%. After liquid nitrogen treatment, α helix increased to 56.5%. ADCC showed that the number of viable fresh cells was 0.24 × 105 and the number of frozen cells was 0.68 × 105. Conclusion: The decrease of immunogenicity of xenotransplanted cells is related to the increase of α-helix ratio in hepatocyte membrane protein.