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目的了解景洪市爱尼族人群阿米巴病流行情况,为阿米巴病监测和防治提供依据,并评价ELISA试剂盒在阿米巴病调查应用的效果。方法以整群随机抽样爱尼族村寨,逐户收集新鲜粪便标本,用观察法、碘液涂片镜检法和TECHLAB第二代溶组织内阿米巴ELISA试剂盒对照检测粪便标本溶内阿米巴。结果镜检278例,E·h/E·d感染为9·71%;ELISA检测366例,E·h感染率为9·29%;镜检和ELISA共同阳性15例,占镜检阳性的55·56%;粪便观察和ELISA检测结果诊断5例为肠阿米巴病,发病率为1·37%,E·h致病率为14·71%。结论景洪爱尼族阿米巴病流行严重,应加强阿米巴病的监测和防治工作,同时积极推广新技术。
Objective To understand the epidemic situation of Amebiasis in the Aini population of Jinghong City and provide the basis for the monitoring and prevention of amebiasis. And evaluate the effect of ELISA kit in the investigation of amebiasis. Methods A sample of fresh stool samples was collected by random sampling in a cluster of Aini Villages. The stool specimens were collected and analyzed by observation method, iodine smear microscopy and TECHLAB second-generation Entamoeba histolytica ELISA kit Miba. Results 278 cases of microscopic examination showed that the infection rate of E · h / E · d was 9.71%. 366 cases were detected by ELISA, the infection rate of E · h was 9 · 29%. The positive results of microscopic examination and ELISA were positive 55.56%. Stool observation and ELISA test results showed that 5 cases were intestinal amoebiasis, the incidence rate was 1.37% and the incidence of E · h was 14.71%. Conclusions Jinghong Aenean is a serious epidemic of Amebiasis. It is necessary to strengthen the monitoring and prevention of amebiasis and actively promote new technologies.