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目的 探讨中国北方地区Ⅰ型对氧磷酯酶 (paraoxonase 1 ,PON1 )基因Gln/Arg1 92遗传多态性与冠心病发病的关系。方法 应用聚合酶链反应 (polymerasechainreaction ,PCR)及限制片段长度多态性 (restrictionfragmentlengthpolymorphisms ,RFLP)技术 ,检测 49例老年冠心病患者和 38例健康老年对照者的PON1 Gln/Arg1 92基因多态性 ,等位基因以A/B表示。结果 冠心病组与健康组比较各基因型分布差异具有显著性意义 (χ2 =6 .35 ,P =0 .0 4 2 )。B等位基因在冠心病组明显增高 (0 .56vs 0 .37)。B等位基因是中国北方地区冠心病发病的危险因素 (OR =2 .1 9,95 %CI:1 .1 9～ 4 .0 5)。结论 PON1基因Gln/Arg1 92遗传多态性与中国北方地区冠心病发病明显相关。该酶切位点多态性具有明显的种族差异
Objective To investigate the relationship between polymorphism of Gln / Arg1 92 paraoxonase type 1 (PON1) gene and the pathogenesis of coronary heart disease in northern China. Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) were used to detect the polymorphisms of PON1 Gln / Arg1 92 in 49 elderly patients with coronary heart disease and 38 healthy elderly controls. The allele is expressed as A / B. Results The distribution of genotypes between CHD group and healthy group was significant (χ2 = 6.35, P = 0.042). B allele in coronary heart disease group was significantly higher (0.56vs 0.37). The B allele was a risk factor for coronary heart disease in northern China (OR = 2.19, 95% CI: 1.19 ~ 4.05). Conclusion The genetic polymorphisms of PON1 Gln / Arg1 92 are significantly associated with the incidence of coronary heart disease in northern China. The restriction enzyme site polymorphism has obvious racial differences