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以南方红豆杉幼茎段和茎尖为外植体,以WPM为基本培养基,单一添加不同浓度的KT、TIBA、ZT和6-BA进行芽诱导培养。结果表明:各自添加0.4mg·L-1KT、3.0mg·L-1TIBA、0.1mg·L-1ZT和0.01mg·L-16-BA时的芽诱导率最高。培养50d的试管微芽中紫杉醇和10-去乙酰基巴卡亭III(10-DABIII)的积累量比天然南方红豆杉嫩枝高2倍以上,试管微芽中10-DABIII比紫杉醇高,且二者均随芽诱导率的增加而增加,当芽诱导率达最高时亦达到最高。TIBA所诱导芽短而粗壮。
The young stem segments and shoot tips of Taxus chinensis were used as explants and WPM was used as the basic medium. KT, TIBA, ZT and 6-BA with different concentrations were added in a single bud culture. The results showed that the bud induction rate was the highest when adding 0.4mg · L-1KT, 3.0mg · L-1 TBA, 0.1mg · L-1ZT and 0.01mg · L-16-BA. Paclitaxel and 10-deacetylbaccatin III (10-DABIII) accumulated more than 2-fold more in shoots of microspores cultured for 50 days than native Taxus chinensis shoots, and 10-DABIII in test tube microtubules was higher than paclitaxel Both of them increased with the increase of bud induction rate, and reached the highest when bud induction rate reached the highest. TIBA induced buds short and thick.