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目的探讨热休克蛋白70(HSP70)体外诱导人外周血单个核细胞(PBMCs)后CC型趋化因子CCL5/RANTES在Vγ9Vδ2 T细胞表达。方法人PBMCs在体外用重组HSP70诱导活化,在第1、4、7、10、14天分别收集细胞,流式细胞术胞内细胞因子分析技术检测表达CCL5/RANTES的Vγ9Vδ2 T细胞百分率,并设结核杆菌低相对分子质量抗原(Mtb-Ag)刺激组作为对照。结果HSP70诱导组分泌CCL5/RANTES的Vγ9Vδ2 T细胞百分率在第1、4、7、10、14天分别为5.9±1.7、16.2±2.5、22.8±3.7、32.4±3.6与57.8±7.2;Mtb-Ag诱导组分别为3.7±2.5、10.5±1.8、11.1±2.3、26.3±3.9与47±4.7;HSP诱导Vγ9Vδ2T分泌CCL5/RANTES的能力比Mtb-Ag强。结论HSP70特异性诱导Vγ9Vδ2 T细胞增殖并分泌CCL5/RANTES,表明二者协同作用,共同参与免疫应答并可能介导某些炎症反应。
Objective To investigate the expression of CC chemokine CCL5 / RANTES in Vγ9Vδ2 T cells induced by heat shock protein 70 (HSP70) induced by human peripheral blood mononuclear cells (PBMCs) in vitro. Methods Human PBMCs were induced by recombinant HSP70 in vitro and the cells were harvested on days 1, 4, 7, 10 and 14. The percentage of Vγ9Vδ2 T cells expressing CCL5 / RANTES was determined by flow cytometry (FCM) Mycobacterium tuberculosis low relative molecular weight antigen (Mtb-Ag) stimulation group as a control. Results The percentages of Vγ9Vδ2 T cells secreting CCL5 / RANTES in HSP70 group were 5.9 ± 1.7, 16.2 ± 2.5, 22.8 ± 3.7, 32.4 ± 3.6 and 57.8 ± 7.2 respectively on the 1st, 4th, 7th, 10th and 14th day. The percentage of Mtb-Ag Induction group were 3.7 ± 2.5, 10.5 ± 1.8, 11.1 ± 2.3, 26.3 ± 3.9 and 47 ± 4.7 respectively; HSP induced the secretion of CCL5 / RANTES by Vγ9Vδ2T stronger than Mtb-Ag. Conclusion HSP70 specifically induced the proliferation of Vγ9Vδ2 T cells and secreted CCL5 / RANTES, indicating that the two interact synergistically to participate in the immune response and may mediate some inflammatory responses.