论文部分内容阅读
目的：探讨蟾毒灵（Bufalin）对人脑胶质瘤 U251细胞生长增殖的影响。方法：采用四甲基偶氮蓝（MTT）比色法检测Bufalin 对人脑胶质瘤 U251细胞增殖的影响；倒置显微镜观察各组细胞数目、形态及活性的变化；膜联蛋白（AnnexinV）碘化丙啶（PI）标记法检测其对细胞凋亡的影响。结果：不同浓度 Bufalin 对 U251细胞增殖活性具有不同程度的抑制作用，在0．001～10μmol·L －1浓度范围内呈剂量和时间依赖性；与对照组相比，Bufalin 给药组对 U251细胞的凋亡率均显著增高（P ＜0．01）。结论：Bufalin 可显著抑制 U251细胞的生长增殖，并在一定范围内呈浓度和时间依赖性，诱导其凋亡。“,”Objective:To explore the effects of Bufalin on the growth and proliferation of human glioma cells U251. Methods:Methyl thiazolyl tetrazolium(MTT)assay was used to detect the effect of Bufalin on the proliferation of human glioma cells U251. An in-verted microscope was used to observe the changes of cell number,morphology and activity. AnnexinV/ PI was used to measure the in-duction of cell apoptosis caused by Bufalin. Results:Bufalin at different concentrations(0. 001 - 10. 0μmol·L - 1 )inhibited the pro-liferation of U251 cells in a dose and time-dependent manner. Compared with that of the control group,the apoptosis rate of Bufalin group was increased significantly(P < 0. 01). Conclusion:Bufalin can inhibit the growth and proliferation of U251 cells in a dose and time-dependent manner,and induce the apoptosis of U251 cells.