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目的探讨护骨素(osteoprotegerin,OPG)基因G1181C位点基因多态性及其血清浓度与急性冠脉综合征(acute coronarysyndrome,ACS)的相关性。方法 134名胸痛患者根据冠状动脉造影及病史分为正常对照组65名和ACS组69名。ELISA法测定入院时血清OPG水平。介质纯化法提取白细胞DNA,聚合酶链式反应(polymerase chain reaction,PCR)扩增包含OPGG1181C位点的DNA片段,连接酶检测反应(ligase detection reaction,LDR)检测PCR产物,识别多态性位点。结果在对照组和ACS组之间,OPG基因G1181C的各基因型频率和分布差异无统计学意义,但ACS组的C等位基因分布频率要显著高于对照组;在对照组和ACS组之间,G1181C各基因型血清OPG浓度差异无统计学意义。结论 OPG基因SNPs G1181C各基因型及其血清浓度与ACS无相关性;C等位基因可能是ACS的致病因子。
Objective To investigate the association of G1181C gene polymorphism and serum concentration of osteoprotegerin (OPG) gene with acute coronarysyndrome (ACS). Methods Totally 134 patients with chest pain were divided into normal control group (n = 65) and ACS group (n = 69) according to coronary angiography and history. Serum OPG levels at admission were measured by ELISA. Leukocyte DNA was extracted by medium purification method, DNA fragment containing OPGG1181C site was amplified by polymerase chain reaction (PCR), ligase detection reaction (LDR) was used to detect PCR products, and the polymorphism sites . Results There was no significant difference in the frequency and distribution of OPG G1181C between control group and ACS group, but the distribution frequency of C allele in ACS group was significantly higher than that in control group. In control group and ACS group There was no significant difference in serum OPG concentration between G1181C genotypes. Conclusion There is no correlation between genotypes and serum concentrations of OPG SNPs G1181C and ACS. C allele may be the causative agent of ACS.