生物素-抗生物素蛋白系统是近年来生物领域中发展起来的一种新型的分离与检测系统。目前,该系统在国外已被广泛地应用于临床微量活性物质的检测、免疫学的临床诊断、抗体抗原大分子物质的纯化、细胞表面组分的定位检测以及临床免疫治疗等方面的基础研究。本文着重对抗生物素蛋白的提取分离及其物化特性,生物素各种衍生物的制备以及达一系统在生物学领域中的应用作一较为详细的介绍。 Biotin-avidin system is a new type of separation and detection system developed in the biological field in recent years. At present, the system has been widely used in foreign countries for the detection of clinical trace active substances, the clinical diagnosis of immunology, the purification of antibody antigen macromolecules, the localization of cell surface components and clinical immunotherapy. This article focuses on the extraction and separation of biotin and its physicochemical properties, the preparation of various derivatives of biotin, and a system in the field of biology for a more detailed introduction.