目的观察急性心肌缺血期大鼠丘脑束旁核神经元μ1阿片受体mRNA不同时间点表达的变化,以探讨μ1阿片受体在丘脑束旁核痛觉整合和调制中的作用。方法体重260～280g的健康成年雄性SD大鼠36只,随机分为两大组对照组,即非冠状动脉扎闭(noncoronaryarteryocclusion、C组)组和扎闭冠脉(coronaryarteryocclusion,CAO组)组。每组又分为T1(CAO1h)、T2(CAO3h)、T3(CAO6h)三个时点。对照组仅在开胸后冠状动脉左前降支下穿线不予结扎;CAO各组则扎闭冠状动脉左前降支。在预定的时点处死动物,取含有大鼠丘脑束旁核的脑片行原位杂交。杂交结果检测采用IDA2000数码显微图像分析系统进行半定量分析。结果CAO后1h、3h、6h大鼠丘脑束旁核神经元μ1阿片受体mRNA的表达均较对照组各时点明显增强(P<0.05),对照组各时点表达无明显差异,而CAO组则随着缺血时间的延长表达有逐渐增加的趋势,在CAO后6h表达最强(P<0.05)。结论急性心肌缺血可诱发大鼠丘脑束旁核神经元μ1阿片受体mRNA表达增强,μ1阿片受体参与急性心肌缺血伤害性刺激在丘脑束旁核的调制。 Objective To investigate the changes of the expression of μ1 opioid receptor mRNA in parafascicular nucleus at different time points after acute myocardial ischemia in rats and to explore the role of μ1 opioid receptors in the hyperalgesia and modulation of paraventricular nucleus of thalamic nucleus. Methods Thirty-six healthy adult male Sprague-Dawley rats weighing 260-280g were randomly divided into two groups: control group (non-coronary artery occlusion group C) and coronary artery occlusion (CAO group). Each group is divided into T1 (CAO1h), T2 (CAO3h), T3 (CAO6h) three time points. The control group was ligated only after the thoracotomy of the left anterior descending coronary artery; the CAO groups closed the left anterior descending coronary artery. The animals were sacrificed at a predetermined time point, and the brain slices containing the thalamic parafascicular nucleus were in situ hybridized. Hybridization test using IDA2000 digital microscope image analysis system for semi-quantitative analysis. Results The expression of μ1 opioid receptor mRNA in parafascicular nucleus at 1h, 3h, 6h after CAO administration was significantly higher than that in control group at each time point (P <0.05), but there was no significant difference in CAO The group showed a trend of increasing gradually with the prolongation of ischemia time, and the expression was the highest 6h after CAO (P <0.05). Conclusions Acute myocardial ischemia can induce neuronal expression of μ1 opioid receptor mRNA in parafascicular nucleus of the thalamus in rats. The modulation of μ1 opioid receptor is involved in the parafascicular nucleus of the thalamic nucleus in acute myocardial ischemic injury.