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背景:传统观点认为癫痫是大脑不同区域内神经元出现异常兴奋引起的复杂神经行为紊乱现象。而对星形胶质细胞在癫痫发病中的作用目前研究较少。目的:研究戊四氮诱导大鼠癫痫发作后延髓内脏带内神经元和星形胶质细胞的反应。设计:随机对照的实验研究。地点和材料:实验在南方医科大学珠江医院神经外科实验室和解放军第四军医大学全军神经科学研究所完成。成年健康SD大鼠14只,体质量180～220g,清洁级,由解放军第四军医大学实验动物中心提供。干预:用抗Fos蛋白、抗酪氨酸羟化酶和抗胶质原纤维酸性蛋白的三重免疫荧光组织化学方法结合激光共聚焦显微镜技术,显示癫痫发作1h后延髓内脏带内反应性神经元与星形胶质细胞的分布。主要观察指标:对延髓内脏带内Fos,胶质原纤维酸性蛋白和抗酪氨酸羟化酶阳性细胞的分布及胶质原纤维酸性蛋白阳性星形胶质细胞与神经元的关系进行观察。结果:延髓内脏带内的Fos阳性神经元和胶质原纤维酸性蛋白阳性星形胶质细胞明显增多,三重免疫组化方法显示反应性神经元(Fos阳性)与反应性星形胶质细胞(胶质原纤维酸性蛋白阳性)关系密切,发现3种不同标记的“神经元-星形胶质细胞复合体”:即抗酪氨酸羟化酶+/Fos+/胶质原纤维酸性蛋白+三标记复合体、抗酪氨酸羟化酶+/胶质原纤维酸性蛋白+/Fos
Background: The traditional view that epilepsy is a complex neuronal behavior disorder caused by abnormal excitability of neurons in different regions of the brain. The role of astrocytes in the pathogenesis of epilepsy is currently less studied. OBJECTIVE: To study the response of pentylenetetrazole-induced neurons and astrocytes in the medulla oblongata after seizure in rats. Design: Randomized controlled experimental study. Location and Materials: Experiments were performed at the Department of Neurosurgery, Zhujiang Hospital, Southern Medical University and the Institute of Neuroscience, Fourth Military Medical University of PLA. Fourteen adult healthy SD rats, body weight 180 ~ 220g, clean level, provided by the Fourth Military Medical University Experimental Animal Center. Intervention: Triple immunofluorescence histochemistry with anti-Fos protein, anti-tyrosine hydroxylase and anti-glial fibrillary acidic protein combined with confocal laser scanning microscopy showed that in the medullary visceral zone of reactive neurons and Astrocyte distribution. MAIN OUTCOME MEASURES: The distribution of Fos, glial fibrillary acidic protein and anti-tyrosine hydroxylase positive cells in medullary visceral zone and the relationship between glial fibrillary acidic protein positive astrocytes and neurons were observed. Results: Fos positive neurons and glial fibrillary acidic protein positive astrocytes in medullary visceral zone were significantly increased. Triple immunohistochemical staining showed that reactive neurons (Fos positive) and reactive astrocytes Glial fibrillary acidic protein positive) was closely related to the discovery of three different labeled “neuronal - astroglial complex”: the anti-tyrosine hydroxylase + / Fos + / glial fibrillary acidic protein + tris Marker complex, anti-tyrosine hydroxylase + / glial fibrillary acidic protein + / Fos