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采用反相微乳液纳米粒子合成方法制备了具有光致化学发光活性的C60/SiO2/Chitosan复合纳米粒子。光致化学发光研究显示该复合纳米粒子与鲁米诺的混合溶液能被波长为400nm的光信号诱导产生鲁米诺的光致化学发光信号,且该发光信号可以被复合纳米粒子表面所吸附的DNA所抑制,据此发现,建立了检测小牛胸腺DNA的光致化学发光新方法。在最佳的实验条件下,光致化学发光信号强度与DNA浓度在1.0×10-9—1.0×10-8mol/L之间表现出了良好线性。
The C60 / SiO2 / Chitosan composite nanoparticles with photoluminescence activity were prepared by reverse microemulsion nanoparticle synthesis. Photoluminescence study shows that the mixed nanoparticle and luminol solution can induce luminol photoluminescence signal by the optical signal with the wavelength of 400 nm, and the luminescent signal can be adsorbed on the surface of the composite nanoparticle DNA inhibition, it was found that the establishment of a new method of detection of calf thymus DNA by photoluminescence. Under the best experimental conditions, the photoluminescence signal intensity and DNA concentration showed a good linearity between 1.0 × 10-9-1.0 × 10-8mol / L.