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免疫荧光间接染色法(简称间接法)是把荧光性染料标记在抗球蛋白抗体上。由于血清蛋白有种的特异性,因此这种标记抗球蛋白抗体(简称标记抗体)可以和同种动物体内免疫的抗血清起反应。测定时,先使被测微生物和抗体起作用,然后加标记抗抗体,与形成的结合物结合,而不至被水冲掉。在紫外光的激发下,通过滤片系统,就能观察到标本的荧光彩色图像。间接染色法的优点是利用一种标记抗体就能与所有在种上相
Indirect immunofluorescence staining (indirect method) is the fluorescent dye labeled anti-globulin antibody. Due to the species specificity of serum proteins, this labeled anti-globulin antibody (labeled antibody) reacts with antisera raised in vivo in the same animal. Determination, the first test microorganisms and antibodies play a role, and then labeled anti-antibodies, and the formation of the combination of binding, and not washed away by water. Fluorescent color images of the specimen can be observed through the filter system when excited by ultraviolet light. The advantage of indirect staining is that using a labeled antibody will work with all species