用小鼠腹腔细胞等作为滋养细胞培养人杂交瘤,国内外均有报道。我们曾建立了人包皮传代细胞株。本文利用我们所建立的人包皮细胞做滋养细胞,并与小鼠腹腔细胞及不加滋养细胞的空白作对照,观察人包皮细胞对杂交瘤克隆生长的影响。 将人包皮细胞和小鼠腹腔细胞分别接种同一96孔板,次日将两株不同的人杂交瘤细胞1B4及1C8稀释至每毫升含10个或50个细胞,每孔接种0.1ml,使每孔分别含1个及5个细胞,经不同时间观察每孔杂交瘤克隆的生长情况,记录有杂交瘤生长的孔数。表1结果表明, The use of mouse peritoneal cells as a trophoblastic culture hybridoma has been reported both at home and abroad. We have established a human foreskin passage cell line. In this study, the human foreskin cells we established were used as trophoblast cells, and compared with mouse peritoneal cells and blanks without trophoblast cells, and the effects of human foreskin cells on the growth of hybridoma clones were observed. The human foreskin cells and mouse peritoneal cells were seeded in the same 96-well plate, and two different human hybridoma cells 1B4 and 1C8 were diluted to 10 or 50 cells per milliliter the next day, and 0.1 ml per well was inoculated. The wells contained 1 and 5 cells, respectively, and the growth of hybridoma clones in each well was observed at different times, and the number of wells in which the hybridomas grew was recorded. The results in Table 1 indicate that