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目的探讨普通光敏剂profrinⅡ纳米化后光动力疗法(PDT)对结肠癌的生长抑制作用。方法超声乳化法构建profrinⅡ纳米微粒,建立人结肠癌LOVO细胞裸鼠种植瘤模型,把荷瘤裸鼠分为对照组：不注射光敏剂,不用激光照射；纳米微粒光敏剂组：注射profrinⅡ纳米微粒悬液,不用激光照射；普通光敏剂PDT组：注射profrinⅡ后3h,激光照射30 min；纳米微粒光敏剂PDT组：profrinⅡ纳米微粒悬液注射后3 h,激光照射30 min。经小鼠尾静脉注入profrinⅡ纳米微粒(30 mg/kg体重),光敏化3 h后用半导体激光仪垂直照射肿瘤30 min(能量密度9J/cm~2)。然后连续观察肿瘤体积的变化及荷瘤裸鼠生存时间,瘤体苏木精-伊红染色病理分析。结果纳米微粒光敏剂PDT组在治疗后早期产生明显的抑制肿瘤增殖作用,瘤体组织坏死,腺腔样结构解离破坏,微血管坏死,血栓形成。纳米微粒光敏剂PDT组与对照组和纳米微粒光敏剂组相比,延命率提高65．2%和58．3%(P＜0．05),抑瘤率增加87．9%和87．5%(P＜0．05)；与普通光敏剂PDT组相比,延命率提高18．8%(P＜0．05),抑瘤率增加56．0%(P＜0．05)。结论纳米微粒光敏剂PDT可明显抑制裸鼠结肠种植瘤生长,延长荷瘤裸鼠生存期。
Objective To investigate the inhibitory effect of photodynamic therapy (PDT) of general photosensitizer profrinⅡ on the growth of colon cancer. METHODS: The profrin Ⅱ nanoparticles were constructed by phacoemulsification and the implanted tumor model of human colon cancer LOVO cells was established. The nude mice bearing tumors were divided into control group, no photosensitizer and no laser irradiation. Nanoparticle photosensitizer group: Suspension, without laser irradiation; ordinary photosensitizer PDT group: 3 hours after injection of profrin Ⅱ, laser irradiation for 30 min; nanoparticle photosensitizer PDT group: 3 hours after injection of profrin Ⅱ nanoparticles suspension, laser irradiation for 30 min. The mice were injected with the profrin Ⅱ nanoparticles (30 mg / kg body weight) through the tail vein of the mice and the tumors were vertically irradiated with semiconductor laser for 30 min (energy density 9 J / cm ~ 2) after 3 h of photosensitization. Then the change of tumor volume and the survival time of tumor-bearing nude mice were observed continuously, and the hematoxylin-eosin staining pathological analysis. Results The nanoparticle photosensitizer PDT group had obvious inhibition of tumor proliferation, tissue necrosis, dissociation of glandular cavity structure, microvascular necrosis and thrombosis in the early stage after treatment. Compared with the control group and the nanoparticle photosensitizer group, the delayed-release rate of the nanoparticle photosensitizer PDT group increased by 65.2% and 58.3% (P <0.05), and the tumor inhibition rate increased by 87.9% and 87.5% % (P <0.05). Compared with PDT group, the longevity rate increased by 18.8% (P <0.05) and the inhibition rate increased by 56.0% (P <0.05). Conclusion Nanoparticle photosensitizer PDT can significantly inhibit the growth of colon xenografts in nude mice and prolong the survival of tumor-bearing nude mice.