论文部分内容阅读
目的研究传统中药枸橘主要成分之一枸橘苷对血管生成的抑制及初步的作用机制。方法用四甲基偶氮唑盐(MTT)法、黏附抑制试验、迁移抑制实验及体内鸡胚绒毛尿囊膜法(CAM法)研究枸橘苷体外抗血管生成作用;用逆转录聚合酶链反应(RT-RCR)方法检测枸橘苷对肿瘤细胞血管内皮生长因子A(VEGF-A)mRNA表达的影响。结果枸橘苷对血管内皮细胞增殖表现出良好的抑制作用,IC50值为(27.8±0.2)μmol·L-1,优于其抑制肿瘤细胞增殖的能力[IC50≥(33.8±0.1)μmol·L-1];在体外该化合物能够明显抑制内皮细胞的黏附和迁移作用,同时体内实验显示该化合物在30μg剂量浓度时能够显著抑制鸡胚绒毛尿囊膜新生血管形成;RT-RCR实验显示枸橘苷在12.5μmol·L-1和25μmol·L-1剂量时显著抑制A549细胞的VEGF-A mRNA表达,且结果具有显著的差异。结论枸橘苷具有抑制新生血管形成作用,其作用机制可能与与抑制血管内皮细胞增殖以及降低肿瘤细胞表达VEGF的量有关。
Objective To study the inhibitory effect of one of the main components of Citrus sinensis, a traditional Chinese medicine, on angiogenesis and its primary mechanism. Methods MTT assay, adhesion inhibition test, migration inhibition test and in vivo chick chorioallantoic membrane method (CAM method) were used to study the anti-angiogenesis effect of pomecronin. (RT-RCR) method was used to detect the effect of citrus on expression of vascular endothelial growth factor A (VEGF-A) mRNA in tumor cells. Results The results showed that puerarin had a good inhibitory effect on the proliferation of vascular endothelial cells with IC50 of (27.8 ± 0.2) μmol·L-1, which was better than that of IC50 (33.8 ± 0.1) μmol·L -1]. In vitro, the compound can significantly inhibit the adhesion and migration of endothelial cells. In vivo experiments showed that the compound could significantly inhibit angiogenesis of chick chorioallantoic membrane at a dose of 30μg. RT-RCR showed that the concentration of citrus Glycosides significantly inhibited the expression of VEGF-A mRNA in A549 cells at doses of 12.5μmol·L-1 and 25μmol·L-1, and the results were significantly different. Conclusion Citrus glycosides can inhibit the angiogenesis of neovascularization, and its mechanism may be related to inhibiting the proliferation of vascular endothelial cells and reducing the amount of VEGF expressed by tumor cells.