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以大豆品种黑农48为接穗,黑籽南瓜为砧木进行嫁接,接穗切面长度设置了5个不同处理。通过嫁接部位组织解剖学分析,深入研究接穗切面长度对嫁接成活率的影响及组织变化规律,探索嫁接诱导植物变异的机理。结果表明:各处理间成活率差异显著,接穗切面长度为1.0 cm时成活率最高,达到80%,愈合的速度也最快;其次是1.3cm。对1.0 cm接穗切面结合部不同时期的电镜动态观察表明,接穗与砧木的愈合过程可分为三个阶段:愈伤组织形成期(1~8 d)、维管组织分化期(9~12 d)、维管组织分化完成期(13~35 d)。研究结果可为大豆远缘嫁接提供理论和实践参考。
Heinon 48 was used as the scion and black seed pumpkin as the rootstock for grafting. The length of the scion was set as 5 different treatments. By anatomical analysis of the grafting site, the effects of the length of the scion on the graft survival rate and the rules of the tissue grafting were investigated in depth to explore the mechanism of grafting induced plant variation. The results showed that there was significant difference in the survival rate among all the treatments. The survival rate was the highest when the length of the scion was 1.0 cm, reaching 80% and the healing rate was the fastest, followed by 1.3 cm. Electron microscopic observation of the 1.0 cm scion scion junction showed that the process of scion and rootstock healing can be divided into three stages: callus formation (1-8 days), vascular tissue differentiation (9-12 days ), The completion of vascular differentiation (13 ~ 35 d). The research results can provide theoretical and practical reference for distant grafting of soybean.