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目的观察枸杞多糖(Lycium barbarum polysaccharide,LBP)对低剂量电离辐射所致雄性大鼠睾丸细胞凋亡的影响。方法 Wistar雄性大鼠84只,随机分为空白对照组、辐射组对照组1、辐射对照组2、辐射对照组3、LBP+辐射组1、LBP+辐射组2、LBP+辐射组3共7组,每组12只,LBP组在每次辐射前给与10 mg/kg LBP干预、其余各组以等量生理盐水灌胃;除空白对照组外,各组进行60Coγ射线局部照射(周一至周五),连续4w,总剂量2.3 Gy/capita。分别于停止照射后1d、7d、14d处死大鼠,。检测脏器系数、精子计数和活力,用RT-PCR,Weston-blot法检测P53和HSP70的m RNA的相对表达量以及蛋白表达。结果 LBP+辐射组1,2,3的睾丸系数较各自辐射对照组增加,以LBP+辐射组3最为明显(P<0.01),但对附性腺器官系数没有明显影响;与辐射对照组1,2,3相比,LBP+辐射组1,2,3的大鼠各组的精子计数和活力明显提高(P<0.01),RT-PCR,Weston-blot法显示LBP+辐射组3的P53m RNA的相对表达量和蛋白表达较辐射对照组3明显降低,而HSP70m RNA的相对表达量和蛋白表达则明显升高(P<0.01);结论 LBP对低剂量电离辐射造成大鼠生殖系统的损伤有一定保护和修复作用。该作用可能是通过减少睾丸细胞的凋亡途径而实现的。
Objective To observe the effect of Lycium barbarum polysaccharide (LBP) on testicular cell apoptosis in male rats induced by low dose ionizing radiation. Methods Eighty-four Wistar male rats were randomly divided into blank control group, radiation group control group 1, radiation control group 2, radiation control group 3, LBP + radiation group 1, LBP + radiation group 2, LBP + radiation group 3 The rats in LBP group were treated with 10 mg / kg LBP before each irradiation, and the other groups were given intragastric administration of normal saline. Except for the blank control group, 60Co γ-rays were given to each group (from Monday to Friday) , Continuous 4w, the total dose of 2.3 Gy / capita. Rats were sacrificed on day 1, day 7 and day 14 after irradiation was stopped. The organ coefficient, sperm counts and viability were detected. The relative expression of m RNA and protein expression of P53 and HSP70 were detected by RT-PCR and Weston-blot. Results The testis coefficient of LBP + irradiation group 1, 2, and 3 were higher than that of their respective radiation control groups, especially LBP + 3 group (P <0.01), but had no significant effect on the numbers of gonadal organs. (P <0.01). The relative expression of P53m RNA in LBP + radiation group 3 was detected by RT-PCR and Weston-blot (P <0.01) .Conclusion LBP can protect and repair the damage of reproductive system in rats caused by low dose ionizing radiation effect. This effect may be achieved by reducing the apoptotic pathways of testicular cells.