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目的探讨甘露糖结合凝集素(MBL)基因多态性与中国汉族人群肺结核发病间的关联。方法采用以医院为基础的病例对照研究设计,对结核病相关危险因素进行问卷调查。联合应用引物序列特异性PCR(PCR-SSP)和序列特异性寡核苷酸探针杂交(PCR-SSOP)等方法对MBL基因H／L、X／Y、P／Q和A／B四个多态性位点进行基因型分析及单倍体型分析。将各位点的基因型或单倍体型在病例组和对照组的分布进行比较,并将有显著作用的环境危险因素纳入进行多因素非条件logistic回归分析。结果 133名肺结核病例和177名对照纳入本研究,多因素分析调整结核病暴露史和疫苗接种史两个因素后,MBL基因的HL和LL基因型与疾病的保护作用显著相关,调整OR值(95％CI)分别为0．54(0．30-0．95)和0．50(0．26-0．96)。MBL基因XB组单倍体型与中国汉族人群肺结核发生的易感性显著相关,调整OR值(95％CI)为1．60(1．07-2．41)。而其余多态性位点,包括X／Y、P／Q和A／B位点与肺结核的发生均无显著性关联。结论中国汉族人群MBL基因多态性可能与汉族人群肺结核发生的易感性显著相关。
Objective To investigate the association between mannose-binding lectin (MBL) gene polymorphism and the incidence of pulmonary tuberculosis in Chinese Han population. Methods A hospital-based case-control study was designed to investigate the risk factors associated with tuberculosis. The combination of PCR-SSP and PCR-SSOP were used to detect the expression of MBL gene H / L, X / Y, P / Q and A / B Polymorphic loci for genotyping and haplotype analysis. The genotypes or haplotypes of each locus were compared in the case group and the control group, and significant environmental risk factors were included in the multivariate non-conditional logistic regression analysis. Results A total of 133 pulmonary tuberculosis cases and 177 controls were included in this study. HL and LL genotypes of MBL gene were significantly associated with disease after adjusting for tuberculosis exposure history and vaccination history by multivariate analysis. Adjusted OR values (95 % CI) were 0.54 (0.30-0.95) and 0.50 (0.26-0.96), respectively. The haplotype of MBL gene XB group was significantly associated with susceptibility to tuberculosis in Chinese Han population. The adjusted odds ratio (95% CI) was 1.60 (1.07-2.41). The remaining polymorphic sites, including X / Y, P / Q and A / B sites and tuberculosis were not significantly associated. Conclusion MBL gene polymorphism in Chinese Han population may be significantly associated with susceptibility to pulmonary tuberculosis in Han population.