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目的观察口服牛视网膜S抗原对人S抗原多肽-35诱发的实验性自身免疫性葡萄膜视网膜炎(EAU)的影响。设计实验性对照研究。研究对象20只雌性纯种Lewis大鼠。方法用人S抗原多肽-35诱发EAU,提纯牛视网膜S抗原诱导口服耐受,分高剂量组(2mg/次)和低剂量组(0.2 mg/次),同时设实验对照组(胎牛血清蛋白)。主要指标EAU发病情况和脾组织白介素-4 (IL-4)和γ-干扰素(IFN-γ)的表达水平。结果口服高剂量组的Lewis大鼠的EAU发病情况较实验对照组有显著减轻(P<0.05),而且口服高剂量组Lewis大鼠脾组织IL-4的表达水平则高于实验对照组(F=4.214,P=0.017)。结论口服高剂量牛视网膜S抗原诱导的免疫耐受可以成功抑制人S抗原多肽-35诱发的实验性自身免疫性葡萄膜视网膜炎。
Objective To observe the effect of oral bovine retinal S antigen on human S antigen peptide-35-induced experimental autoimmune uveitis (EAU). Design experimental control study. Twenty female, purebred Lewis rats were studied. Methods Erythropoietin (EAU) was induced by human S antigen peptide-35, and oral retinal S antigen was purified to induce oral tolerance. The rats were divided into high dose group (2mg / time) and low dose group (0.2mg / ). The main indicators EAU incidence and the level of interleukin-4 (IL-4) and interferon-γ (IFN-γ) in the spleen. Results The incidence of EAU in Lewis rats in oral high dose group was significantly lower than that in experimental group (P <0.05), and the expression of IL-4 in Lewis rats was higher than that in experimental group (F = 4.214, P = 0.017). Conclusion The immune tolerance induced by oral high-dose bovine retinal S antigen can successfully inhibit the experimental autoimmune uveoretinitis induced by human S antigen peptide-35.