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目的:研究黄豆提取物对经戊四唑(pentylenetrazole,PTZ)诱发癫发作的女性荷尔蒙缺失雌性大鼠与正常雌性大鼠的不同作用,以及因性别差异引起的植物雌性激素对行为的影响。方法:雄性Wistar大鼠被随机分为雄性生理盐水组,雄性低、中、高剂量黄豆提取物治疗组,每组8只;雌性Wistar大鼠随机分为假手术生理盐水组,假手术低、中、高剂量黄豆提取物治疗组,去卵巢生理盐水组,去卵巢低、中、高剂量黄豆提取物治疗组,每组8只。去卵巢大鼠在氯胺酮麻醉下行卵巢切除术。分别给予各组大鼠生理盐水及不同剂量黄豆提取物治疗2周后腹腔内注射戊四唑。将大鼠放置在树脂玻璃笼内,记录最小阵挛性癫发作(minial clonic seizure,MCS)潜伏期和强直性阵挛性癫发作(generalized tonic-clonic seizure,GTCS)潜伏期。结果:与雄性生理盐水组大鼠比较,雄性低、中剂量黄豆提取物治疗组的MSC和GTCS潜伏期显著缩短(P<0.05或P<0.01)。雌性假手术大鼠在给予黄豆提取物治疗后,其MSC和GTCS潜伏期没有显著改变。与去卵巢生理盐水组比较,去卵巢低、高剂量黄豆提取物治疗组的MSC和GTCS潜伏期明显缩短(P<0.05或P<0.01)。结论:黄豆的植物雌激素能影响由PTZ诱发的癫发作的轻重程度,但其影响程度与卵巢激素水平有关。机制还有待进一步研究。
OBJECTIVE: To study the different effects of soybean extract on estrogen-deficient female rats and normal female rats induced by pentylenetrazole (PTZ) and the effect of plant estrogen on behavior caused by gender differences. Methods: Male Wistar rats were randomly divided into male saline group, male low, medium and high doses of soybean extract treatment group, each group of eight; female Wistar rats were randomly divided into sham-operated saline group, sham surgery, Medium and high dose of soybean extract treatment group, ovariectomized normal saline group, ovariectomized low, medium and high dose soybean extract treatment group, 8 in each group. Ovariectomized ovariectomized rats under ketamine anesthesia. Rats in each group were given saline and different doses of soybean extract after 2 weeks of treatment of intraperitoneal injection of pentylenetetrazol. The rats were placed in a Plexiglas cage and the incubation period for minimal clonic seizure (MCS) and generalized tonic-clonic seizure (GTCS) were recorded. Results: Compared with male rats in normal saline group, the latency of MSCs and GTCS in male low and medium dose soybean extract treatment groups was significantly shorter (P <0.05 or P <0.01). Female sham rats did not significantly change their latent period of MSC and GTCS after administration of soy extract. Compared with the ovariectomized saline group, the latent period of MSCs and GTCS in the ovariectomized low and high dose soy extract groups was significantly shorter (P <0.05 or P <0.01). CONCLUSIONS: Soybean phytoestrogen can affect the severity of epileptic attacks induced by PTZ, but its effect is related to ovarian hormone levels. Mechanism remains to be further studied.