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目的观察姜黄素对高糖诱导的INS-1胰岛β细胞凋亡的作用,探讨其抗糖尿病作用的可能机制。方法流式细胞术检测细胞凋亡情况;透射电子显微镜观察INS-1细胞的形态学变化;采用激光共聚焦和流式细胞术检测INS-1细胞线粒体膜电位的变化;用Western blotting法检测Bcl-2、Bax蛋白的表达量。结果姜黄素浓度为10μmol/L抗凋亡作用效果最为明显(P<0.05)。与正常组相比,模型组INS-1细胞凋亡率明显升高(P<0.05),形态发生改变,线粒体膜电位降低,Bcl-2蛋白表达明显降低(P<0.05),Bax蛋白表达明显升高(P<0.05),Bcl-2/Bax比值降低。与模型组比较,姜黄素改善凋亡的INS-1细胞的形态,提高线粒体膜电位,降低Bax蛋白表达(P<0.05),增加Bcl-2蛋白表达(P<0.05),Bcl-2/Bax比值升高。结论姜黄素改善高糖诱导的INS-1细胞凋亡状态,其抗凋亡机制与线粒体途径有关。
Objective To observe the effect of curcumin on the apoptosis of INS-1 cells induced by high glucose and to explore the possible mechanism of its anti-diabetic effect. Methods The apoptosis of INS-1 cells was detected by flow cytometry. The morphological changes of INS-1 cells were observed by transmission electron microscopy. The changes of mitochondrial membrane potential of INS-1 cells were detected by laser confocal microscope and flow cytometry. -2, Bax protein expression. Results The anti-apoptotic effect of curcumin at a concentration of 10μmol / L was the most significant (P <0.05). Compared with the normal group, the apoptosis rate of INS-1 cells in model group was significantly increased (P <0.05), and the morphology was changed, the mitochondrial membrane potential was decreased, the expression of Bcl-2 protein was significantly decreased (P <0.05) (P <0.05), Bcl-2 / Bax ratio decreased. Compared with model group, curcumin could improve the morphology of INS-1 cells, increase mitochondrial membrane potential, decrease Bax protein expression, increase Bcl-2 protein expression (P <0.05), and Bcl-2 / Bax Ratios. Conclusion Curcumin can improve the apoptosis of INS-1 cells induced by high glucose, and its anti-apoptotic mechanism is related to mitochondrial pathway.