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目的用实时荧光定量PCR方法对肺部感染危重患者的咽拭子样本和支气管肺泡灌洗液(BALF)进行检测,探讨不同样本对检测结果的影响。方法 450例肺部感染危重患者纳入临床研究,用实时荧光定量PCR方法检测患者咽拭子样本和BALF样本,对检测结果进行比较。结果在450例肺部感染危重患者中,肺炎支原体肺炎(MPP)患者115例,占25.6%。BALF样本检测的阳性率为69.56%,咽拭子样本检测的阳性率为13.04%。肺炎支原体(MP)感染的年龄段主要在20~39和50~59岁,以50~59岁年龄段人数最多。结论 MP是肺部感染主要病原体之一,采集BALF标本用实时荧光PCR检测MPP阳性率更高,是MPP诊断的有效手段。
Objective To detect the throat swab samples and bronchoalveolar lavage fluid (BALF) of critically ill patients with lung infection by real-time fluorescence quantitative PCR and to explore the influence of different samples on the test results. Methods A total of 450 critically ill patients with pulmonary infection were included in the clinical study. The throat swab samples and BALF samples were detected by real-time fluorescence quantitative PCR, and the results were compared. Results Of the 450 critically ill patients with pulmonary infection, 115 patients (25.6%) had mycoplasma pneumoniae pneumonia (MPP). The positive rate of BALF was 69.56% and the positive rate of throat swab was 13.04%. Mycoplasma pneumoniae (MP) infection in the age groups mainly in the 20 to 39 and 50 to 59 years old, to 50 to 59 age group the largest number. Conclusion MP is one of the major pathogens of pulmonary infection. The positive rate of MPP in BALF specimens collected by real-time fluorescence PCR is higher, which is an effective method for MPP diagnosis.