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目的与方法 : 应用随机引物 PCR(简称 AP- PCR或 PAPD)和 set1、set2两基因 PCR方法分析了 71株F2 a志贺菌的基因多态性。 结果 :两种引物的 AP- PCR中 ,引物 12将 71株 F2 a志贺菌分为两种不同的基因型 ,引物 17和 set1、set2两基因 PCR将所有菌株分为四种不同的基因型 ,两种方法结合则可分为七种不同的基因型 ;其中 6 5株 F2 a志贺菌基因型相同 ,其余 6株各为独立的型别。本研究表明 AP- PCR和 set1、set2两基因 PCR为志贺菌基因多态性分析的有效手段 ,二者结合则更为完善 ;研究结果还表明 ,在我国南北不同地区不同时间分离到的无论是暴发株还是散发株 ,均具有相同的优势克隆。 结论 :上述菌株是引起国内菌痢散发和暴发的主要基因型 ,是流行病学研究和防治的重点
Objective and Methods: The genetic polymorphisms of 71 strains of F2 Shigella were analyzed by random primer PCR (AP-PCR or PAPD) and set1 and set2 two-gene PCR. Results: In AP-PCR with two primers, 71 strains of F2a were divided into two different genotypes. Primer 17 and set1 and set2 genes were used to divide all strains into four different genotypes The combination of the two methods can be divided into seven different genotypes; of which 65 strains of F2 a Shigella genotypes the same, the remaining 6 were independent types. This study showed that AP-PCR and set1, set2 two genes PCR effective means for the analysis of Shigella genetic polymorphism, the combination of the two is more perfect; the results also show that in different parts of our country at different times separated regardless of whether Are outbreaks or sporadic strains, all with the same advantages of cloning. Conclusion: The above strains are the main genotypes that cause the domestic bacteriosis to disperse and outbreak, which is the focus of epidemiological research and prevention and treatment