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种子萌发期,筛选、鉴定高粱抗旱材料对挖掘种子萌发期抗旱基因以及培育抗旱性更强的高粱品种都具有重要意义.本研究采用聚乙二醇(PEG6000)模拟干旱胁迫,设置0(CK)、5％、10％、15％、17.5％、20％、 22.5％和25％ 8个不同浓度梯度PEG6000对粒用高粱BTx623和甜高粱Rio进行干旱处理,确定PEG6000初步筛选浓度,然后利用PEG6000初步筛选浓度对全球收集的396份高粱进行初步筛选,筛选出较抗旱的材料逐渐加大选择压力,最终筛选出高抗旱性的高粱材料.基于以上试验,确定了大批材料初步筛选时 PEG6000浓度为15％较为适宜.396份高粱材料经过15％ PEG6000初步筛选时,相对出苗率达到80％以上的有89份,逐步加大筛选压力,最终筛选出4份抗旱能力很强的高粱材料,其中3份来自中国的粒用高粱,1份来自东亚的粒用高粱.随着处理浓度的增加,高粱抗旱性和耐盐性两者之间的相关性存在不确定性.“,”At the seed germination stage, screening and identification the drought-resistant materials, which is great significance for exploring drought-resistant genes and cultivating the drought-resistant varieties. In this research, we set, 0 (CK), 5％, 10％, 15％, 17.5％, 20％, 22.5％ and 25％, 8 different concentration gradients PEG6000 for drought treatment of grain sorghum BTx623 and sweet sorghum Rio, which was aim at determine the initial screening concentration of PEG6000. Then, 396 sorghum materials, from the whole world, were treated by the PEG6000 initial screening concentration to screen drought-resistant materials, and then gradually increase selection pressure to screen stronger drought-resistant materials. Based on the above test, we confirmed that 15％ PEG6000 was appropriate for initial screening of a large number of materials. 396 sorghum materials were treated by 15％ PEG6000, 89 higher drought-resistant materials were obtained, which the relative seedling-emergence rates were more than 80％. Step up the screening pressure, 4 mostly stronger drought-resistant materials were obtained. 3 materials of them were from China, 1 material were from East Asia, which were all grain sorghum. With the increasing of treatment concentration, the correlation between drought resistance and salinity tolerance of sorghum might exist uncertainty.