目的利用A型、B型流感病毒荧光RT-PCR快速检测法,对流感病毒作快速诊断,用细胞分离法能及时取得病毒株,掌握流感流行及毒株的变异,分析预测流感在我区流行的情况,为流感防治工作提供科学依据。方法A型、B型流感病毒荧光RT-PCR快速检测法和常规狗肾传代细胞(MDCK)法分离流感病毒并进行毒株鉴定。结果B型流感病毒荧光RT-PCR为阳性;细胞病毒分离培养也确认为B型(Victoria系)流感。结论该起疫情是由B型(Victoria系)流感病毒引的急性上呼吸道病。 OBJECTIVE: To rapidly diagnose influenza virus by Fluorescent RT-PCR rapid detection of influenza A and B viruses and to obtain the virus strains by cell isolation in time, to master the variation of influenza and virus strains, to predict the prevalence of influenza in our region For the flu prevention and control work to provide a scientific basis. Methods Influenza A virus and influenza B virus were detected by fluorescent RT-PCR and conventional canine kidney cell line (MDCK) method to isolate and identify influenza virus. Results Fluorescent RT-PCR of influenza B virus was positive. Cytomegalovirus isolation and culture was also confirmed as type B (Victoria-type) flu. Conclusion The outbreak was an acute upper respiratory disease induced by influenza B (type B) virus.