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目的 检测活动性狼疮性肾炎 (LN)患者外周血和肾组织内浸润T淋巴细胞受体 (TCR)基因亚家族的限制性取用 ,以探讨体内自身反应性T细胞克隆存在的证据。方法 用半定量逆转录聚合酶链反应 (RT PCR)的方法分析 18例活动性LN患者和 12名正常对照者的T细胞受体Vβ亚家族的部分基因表达。结果 活动性LN患者外周血T淋巴细胞受体Vβ8基因表达明显高于正常对照组(0 .6 7± 0 .11vs 0 .36± 0 .0 3,P <0 .0 0 1) ,肾活检组织中浸润淋巴细胞Vβ8基因的表达也明显高于TCRVβ1、TCRVβ11、TCRVβ2 0这些TCRVβ家族的其他成员 (P <0 .0 0 1) ,并呈现出以TCRVβ8为优势克隆的T细胞的大量增殖和聚集。结论 在LN的自身免疫性损伤中 ,存在具同一特征 (TCRVβ8)自身反应性T细胞的克隆聚集、增殖 ,这一发现提示其在LN中可能具有致病作用
Objective To investigate the restricted access of infiltrating T lymphocyte receptor (TCR) gene subfamily in peripheral blood and renal tissues in patients with active lupus nephritis (LN) to explore the evidence of the existence of autoreactive T cell clones in vivo. Methods The partial gene expression of T cell receptor Vβ subfamily in 18 active LN patients and 12 normal controls was analyzed by semiquantitative reverse transcription polymerase chain reaction (RT PCR). Results The expression of Vβ8 in peripheral T lymphocyte of patients with active LN was significantly higher than that of the control group (0.67 ± 0.11 vs 0.36 ± 0.30, P <0.01). The renal biopsy The expression of Vβ8 in infiltrating lymphocytes was also significantly higher in T lymphocytes than in TCRVβ1, TCRVβ11 and TCRVβ20 (P <0.01), and showed a significant proliferation of T cells transfected with TCRVβ8 Gather together. Conclusions In autoimmune lesions of LN, clonal aggregation and proliferation of autoreactive T cells with the same feature (TCRVβ8) exist and this finding suggests that it may have a pathogenic role in LN