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以陆地棉(Gossypium hirsutum L.)中棉所8号和海岛棉(Gossypium barbadense L.)Pima90-53组配衍生的214个单株的F2群体为材料,构建了包含110个SSR标记和65个AFLP标记的遗传连锁图谱。该图谱共包括42个连锁群,连锁群长度为4.5～147.3cM,包括2～22个分子标记,标记间平均距离为11.6cM,总长为2030cM,约占棉花全基因组的40.6%。应用复合区间作图法分析该组合的F2单株和F2:3家系纤维品质性状,共得到25个纤维品质数量性状基因座(QTL),其中5个与纤维长度相关,分布在Chr.21、Chr.15、LG2和LG12上,可解释表型变异的10.2%～35.8%;4个与整齐度相关,分布在Chr.21、LG9、LG18和LG12上,可解释表型变异的12.6%～36.6%;7个与马克隆值相关,分布在Chr.9、LG1、LG9、LG20和LG12上,可解释表型变异的11.5%～26.1%;7个与断裂比强度相关,分布在Chr.21、Chr12、Chr.8、LG1、LG4和LG10上,可解释表型变异的16.5%～52.8%;2个与伸长率相关,分布在Chr.9和Chr.21上,可解释表型变异的18.1%和27.1%。LG9、LG12和Chr.21上存在QTL聚集区。
F2 population of 214 individuals derived from a combination of Gossypium hirsutum L. and Gossypium barbadense L. Pima90-53 was used as materials to construct a cluster of 110 SSR markers and 65 AFLP marker genetic linkage map. The map consisted of 42 linkage groups, ranging from 4.5 to 147.3 cM in linkage group, including 2 to 22 molecular markers with an average distance of 11.6 cM and a total length of 2030 cM, accounting for 40.6% of the total genome of cotton. The fiber quality traits of F2 and F2: 3 lines were analyzed by composite interval mapping. A total of 25 QTLs were obtained, of which 5 were related to fiber length and distributed in Chr.21, Chr.15, LG2 and LG12 explained 10.2% ~ 35.8% of the phenotypic variation; 4 were related to uniformity and distributed on Chr.21, LG9, LG18 and LG12, which explained 12.6% ~ 36.6%; 7 were related to micronaire value and distributed on Chr.9, LG1, LG9, LG20 and LG12, which explained 11.5% -26.1% of phenotypic variation; 7 were related to fracture intensity and distributed in Chr. 21, Chr12, Chr.8, LG1, LG4 and LG10 explained 16.5% -52.8% of the phenotypic variation. The two were related to the elongation and distributed on Chr.9 and Chr.21, which could explain the phenotype 18.1% and 27.1% of variance. QTL aggregates exist on LG9, LG12 and Chr.21.