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A fast,sensitive,and reliable method for the determination of sulfite(SO32-)in fresh water and seawater samples was developed.The proposed method was based on the reaction of o-phthalaldehyde(OPA)-sulfite-NH3 in alkaline solution,with flow injection analysis and fluorescence detection.The experimental parameters were investigated in pure water and seawater matrixes.The detection limits(S/N = 3)were 0.006 μmol/L in pure water and 0.018 μmol/L in seawater for SO32-.The method was successfully applied to analyze SO32-in the samples of rain water and flue gas desulfurization seawater.