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目的探讨p53腺病毒(简称Ad-p53)注射液联合中药得力生及消癌平治疗小鼠腹水瘤的协同作用。方法建立昆明小鼠H22肝癌腹水瘤模型,建模48h后分组分别给予Ad-p53、中药得力生和消癌平或两药联用。用药1周后检测体重、腹围、腹水量、腹水瘤细胞计数,流式细胞仪测定瘤细胞凋亡百分率以及细胞周期情况。结果Ad-p53、得力生、消癌平单药治疗组,除体重以外的上述指标与对照组比较均有统计学差异(P<0.05),Ad-p53+得力生和Ad-p53+消癌平组的腹围增长率、腹水量、瘤细胞计数、晚期细胞凋亡率均明显少于各单药治疗组(P<0.05)。结论腹腔注射Ad-p53、得力生和消癌平均可抑制H22细胞生长并减少腹水形成,Ad-p53与中药得力生、消癌平联合腹腔内给药对治疗腹水瘤有协同作用。
Objective To investigate the synergetic effect of p53 adenovirus (Ad-p53) injection combined with traditional Chinese medicine Deli and Xiao-ping on mice ascites tumor. Methods Kunming mouse H22 hepatocellular carcinoma ascites tumor model was established, 48 hours after the model group were given Ad-p53, effective Chinese medicine and Xiaopeng Ping or a combination of the two drugs. After 1 week, the body weight, abdominal circumference, ascites volume, ascites tumor cell count, percentage of tumor cell apoptosis and cell cycle were determined by flow cytometry. Results Ad-p53, effective Lisheng, Xiaofanping monotherapy group, in addition to the weight of the above indicators compared with the control group were statistically significant (P <0.05), Ad-p53 + effective and Ad-p53 + The rate of abdominal circumference, the amount of ascites, the number of tumor cells and the rate of apoptosis in late stage were significantly less than those in the single drug treatment group (P <0.05). Conclusion Intraperitoneal injection of Ad-p53 can effectively inhibit the growth of H22 cells and reduce the formation of ascites due to the suppressive and anti-cancerous effects. Synergistic effect of Ad-p53 and traditional Chinese medicine (TXL) and Xiaowanping combined with intraperitoneal injection on the treatment of ascites tumor was observed.