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用一对专化于印度腥黑穗病菌的引物Ｔ１１７Ｍ１（５＇－ＴＣＣＣＣＴＴＧ－ＧＡＴＣＡＧＡＡＣＧＴＡ－３＇）和Ｔ１１７Ｍ２（５＇－ＡＧＡＡＧＴＣＴＡＡＣＴＣＣＣＣＣＣＴＣＴ－３＇）可特异地扩增印度腥黑穗病菌产生一段８２５ｂｐ的产物，而稻粒黑粉病菌则不能被扩增。实验还表明，用聚合酶链反应（ＰＣＲ）方法检测灵敏度可达到１００个未萌发的冬孢子，这为进口粮印度腥黑穗病菌的检疫提供了有力工具。
Taraxacum mirifilus could be specifically amplified with a pair of primers T117M1 (5’-TCCCCTTG-GATCAGAACGTA-3 ’) and T117M2 (5’-AGAAGTCTAACTCCCCCCTCT-3’) specialized for M. indica, producing an 825 bp Products, and rice powdery mildew bacteria can not be amplified. Experiments also showed that the sensitivity of PCR-based assays could reach 100 non-germinating teliospores, which provided a powerful tool for the quarantine of the import of Toutlet Indian smut.