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目的通过检测细菌性阴道病(BV)患者阴道分泌物中抗氧化酶、脂质过氧化物、阴道乳酸杆菌的状况及相互关系,探讨氧化应激与细菌性阴道病发病机制的关系。方法分泌物涂片观察病原微生物类型并统计乳酸杆菌的数量;阴道分泌物制成酶检测储备液,应用试剂盒检测阴道炎分泌物中脂质过氧化物丙二醛(MDA)、过氧化氢(H2O2),以及抗氧化物酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及维生素C(VC)的活性或含量。结果 BV在本组研究中发病率为33.5%;BV患者阴道分泌物pH值显著升高,乳酸杆菌检出率降低,脂质过氧化物MDA、H2O2水平升高,抗氧化物酶CAT、SOD、VC水平均显著低于对照组,接受治疗后各项指标均恢复至正常水平。结论氧化应激参与并影响了BV的发病过程,通过改变阴道内环境氧化应激状况治疗BV具有前景。
Objective To investigate the relationship between oxidative stress and pathogenesis of bacterial vaginosis by detecting the status of anti-oxidative enzymes, lipid peroxides and lactobacilli in vaginal secretions of patients with bacterial vaginosis (BV). Methods Secretion smears were used to observe the types of pathogenic microorganisms and count the number of lactobacilli. Vaginal secretions were used to prepare enzyme assay stock solution. Lipid peroxides malondialdehyde (MDA), hydrogen peroxide (H2O2), and the activities or contents of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and vitamin C (VC). Results The incidence of BV in this study was 33.5%. The BV of vaginal secretions was significantly increased, the detection rate of lactobacilli was decreased, the levels of MDA and H2O2 were increased, and the activities of CAT and SOD , VC levels were significantly lower than the control group after treatment, the indicators were restored to normal levels. Conclusion Oxidative stress is involved in and affects the pathogenesis of BV. It is promising to treat BV by changing the intra-vaginal oxidative stress.