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目的检测苯扎贝特干预后小鼠血脂浓度,以及血清、肝脏及粪便中~3H-胆固醇占经腹腔注射~3H-胆固醇总量的百分比,探讨苯扎贝特对小鼠体内胆固醇逆转运的影响。方法 35只C57BL/6小鼠随机分为5组,分别给予普通饲料、不同剂量的苯扎贝特(0.1%、0.25%、0.5%、0.75%)添加普通饲料喂养4周后,腹腔注射经acLDL及~3H-胆固醇处理过的小鼠巨噬细胞悬液(0.5 mL/鼠,细胞数达5.0×10~6),单独笼养24h后取血,酶法测定血脂;测定血清、肝脏和粪便中的~3H-胆固醇含量(占注射总量的百分比)。结果不同剂量苯扎贝特(0.1%、0.25%、0.5%、0.75%)干预小鼠后,高密度脂蛋白胆固醇(HDL-C)升高并呈剂量相关性趋势。0.75%苯扎贝特组HDL-C水平较对照组明显增高,增幅为30.3%;血清甘油三酯(TG)水平较对照组明显降低,降幅为40.3%。苯扎贝特干预各组(0.1%、0.25%、0.5%、0.75%)小鼠血清、肝脏、粪便~3H的总含量显著增多,苯扎贝特干预组(0.1%、0.25%、0.5%、0.75%)小鼠粪便~3H-胆固醇含量较对照组增幅分别为+1.11,+1.40,+1.60,+1.70倍。结论苯扎贝特剂量依赖性增加HDL-C和降低TG,能加速胆固醇由粪便清除,促进体内胆固醇逆转运,利于动脉粥样硬化的防治。
Objective To detect the concentration of 3H-cholesterol in serum, liver and feces of mice after bezafibrate intervention and the percentage of ~ 3H-cholesterol in peritoneal injection to investigate the effect of bezafibrate on cholesterol reverse transport in mice influences. Methods 35 C57BL / 6 mice were randomly divided into 5 groups, which were fed with normal diet. The animals were fed with normal diet after 4 weeks of different doses of bezafibrate (0.1%, 0.25%, 0.5%, 0.75%), acLDL and ~ 3H-cholesterol-treated mouse macrophage suspension (0.5 mL / mouse, the number of cells reached 5.0 × 10 ~ 6), 24h separate blood and enzymatic determination of serum lipids; serum, liver and 3H-cholesterol content in feces (% of total injected volume). Results After the different doses of bezafibrate (0.1%, 0.25%, 0.5%, 0.75%) were administered to mice, HDL-C increased and showed a dose-dependent trend. The level of HDL-C in 0.75% bezafibrate group was significantly higher than that in control group (30.3%). The level of serum triglyceride (TG) was significantly lower than that in control group (40.3%). The total content of ~ 3H in serum, liver and stool of mice in the group of bezafibrate (0.1%, 0.25%, 0.5%, 0.75% , 0.75%) mice stool ~ 3H-cholesterol content increased by + 1.11, + 1.40, + 1.60, + 1.70 times than the control group respectively. Conclusion Bezafibrate can increase HDL-C and decrease TG in a dose-dependent manner, which can accelerate the clearance of cholesterol from the stool, promote the reverse transport of cholesterol in the body and promote the prevention and treatment of atherosclerosis.