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大量研究表明,肿瘤细胞增殖活性与其生长、浸润、复发、转移等生物学行为及预后密切相关.目前,用于细胞增殖活性检测的方法较多,其意义尚有争议.现将有关研究方法及其意义作一综述.1 核发裂计数核分裂计数(mitotic count,MC)指计数每10个高倍视野中核分裂的数目.可对常规固定处理的组织切片,经H-E染色后进行 观察并计数,是最简单的评价细胞增殖状态的方法.在许多肿瘤的病理学及临床研究中,长期被用作辅助诊断及预测预后的手段.但是,其检测结果可受许多因素影响:如组织固定方法、显微镜视野大小、肿瘤异质性等.因此,有作者建议采用核分裂
A large number of studies have shown that the proliferation activity of tumor cells is closely related to their biological behaviors such as growth, infiltration, recurrence, metastasis, and prognosis. At present, there are many methods used for the detection of cell proliferation activity, and its significance is still controversial. Now the relevant research methods and Its significance is reviewed.1 Nuclear split count Mitosis count (MC) refers to counting the number of mitoses in every 10 high-power fields of view. Conventional fixed tissue sections can be observed after HE staining and counted. A simple method for evaluating the state of cell proliferation has long been used as a means of assisting diagnosis and predicting prognosis in the pathology and clinical studies of many tumors. However, the detection results may be influenced by many factors such as tissue fixation method, microscope view Size, tumor heterogeneity, etc. Therefore, there are authors suggesting the use of nuclear fission