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目的:初步探讨抗苗勒氏管激素(AMH)及其受体Ⅱ(AMHRⅡ)的基因多态性与卵巢过度刺激综合征(OHSS)的关系。方法:采用聚合酶链反应(PCR)和DNA测序法,分别检测27例OHSS患者和22例促排卵后非OHSS患者(对照组)的AMH及其受体AMHRⅡ的基因外显子DNA序列,行单核苷酸多态性(SNP)分析。结果:OHSS组AMH的第1外显子146位G>T、第2外显子134位G>A基因型分布与对照组比较,有统计学差异(P<0.05)。OHSS组AMH基因的第1外显子303位G>A基因型与对照组比较,无统计学差异(P>0.05)。OHSS组和对照组AMHRⅡ基因的1~11号外显子均未检测出SNP突变。结论:AMH基因多态性可能是导致卵巢对外源性激素敏感性增强、OHSS发病的因素之一。
Objective: To investigate the relationship between anti-Müllerian hormone (AMH) and its receptor Ⅱ (AMHRⅡ) gene polymorphisms and ovarian hyperstimulation syndrome (OHSS). Methods: The exon DNA sequences of AMH and its receptor AMHR Ⅱ in 27 patients with OHSS and 22 patients with non-OHSS after ovulation induction (control group) were detected by polymerase chain reaction (PCR) and DNA sequencing. Single nucleotide polymorphism (SNP) analysis. Results: There was a significant difference (P <0.05) between the distribution of 146 G> T exon 1 and 134 G> A genotype of AMH in OHSS group compared with the control group. OHSS group AMH gene exon 1 303 G> A genotype compared with the control group, no significant difference (P> 0.05). No SNP mutation was detected in exon 1 to exon 11 of AMHR Ⅱ gene in OHSS group and control group. CONCLUSION: AMH gene polymorphism may be one of the factors leading to the increased sensitivity of ovary to exogenous hormones and OHSS.