论文部分内容阅读
致病性钩端螺旋体是广泛分布的人兽共患病——钩端螺旋体病的致病菌。多种致病性钩端螺旋体能产生不同种类的溶血素,其对红细胞的裂解及对哺乳动物细胞的毒性作用与其他致病微生物溶血素的细胞毒性有相似之处。最近,对强毒株问号状钩端螺旋体黄疽出血型赖株基因组测序及基因诠释工作已经完成,首次发现其中至少有9个溶血素基因,这些基因在大肠杆菌BL21(DE3)pLysS中克隆、表达,成功地验证了它们的溶血活性。如此多的溶血素基因同时存在于钩端螺旋体基因组中,说明溶血素基因和钩端螺旋体的致病性密切相关。
Pathogenic Leptospira is a widely distributed zoonosis - a pathogen of leptospirosis. A variety of pathogenic Leptospira can produce different types of hemolysin, its role in the cleavage of red blood cells and the toxicity of mammalian cells and other pathogenic microbial hemolysin cytotoxic similarities. Recently, the genome sequence and gene annotation of the L. parahaemolyticus Jaundiceus parahaemolyticus strain have been completed. For the first time, at least nine hemolysin genes were found in these strains. These genes were cloned in E. coli BL21 (DE3) pLysS, Expression, successfully verified their hemolytic activity. So many hemolysin genes exist in the genome of Leptospira, indicating that hemolysin gene and Leptospira pathogenicity are closely related.