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目的 观察抗大肠杆菌卵黄免疫球蛋白 (IgY)对大鼠放射性肠炎的防护作用。方法 将 30只大鼠随机分为正常对照组 (A组 )、照射对照组 (B组 )和IgY保护组 (C组 )。大鼠全腹照射10 0 0cGy。C组自照射前 1d开始灌服IgY。 4d后处死大鼠 ,观察肠道细菌移位情况、血中内毒素水平及小肠粘膜病理形态学改变。结果 A组无细菌移位 ,B组细菌移位最为明显 (96 7% ) ,C组细菌移位 (13 3% )远不及B组明显 ;A组内毒素含量极低 (0 0 0 1EU ml) ,B组内毒素含量明显升高 (0 82 9EU ml) ,C组内毒素含量 (0 2 4 9EU ml)明显低于B组 ;A组肠粘膜正常 ,B组肠粘膜绒毛水肿 ,炎性细胞浸润 ,部分上皮细胞脱落 ,C组绒毛轻度水肿 ,未见明显细胞脱落。结论 全腹照射能明显损伤小肠粘膜 ,引起细菌移位和内毒素血症 ,抗大肠杆菌IgY能明显减轻射线对小肠粘膜的损伤。
Objective To observe the protective effect of anti-E.coli yolk immunoglobulin (IgY) on radiation enteritis in rats. Methods Thirty rats were randomly divided into normal control group (group A), irradiation control group (group B) and IgY protection group (group C). Whole abdominal irradiation of rats 100cGy. Group C received 1 day before irradiation irrigated with IgY. After 4 days, the rats were sacrificed to observe the intestinal bacterial translocation, blood endotoxin levels and intestinal mucosal pathological changes. Results The bacterial translocation in group A was the most obvious (96 7%). The bacterial translocation in group C was far less than that in group B (13 3%). The endotoxin content in group A was extremely low (0 0 0 1 EU U ml ), The level of endotoxin in group B was significantly increased (0 82 9EU ml), the level of endotoxin in group C (0 2 49 9UU ml) was significantly lower than that in group B. The intestinal mucosa was normal in group A, the villus edema in group B Cell infiltration, some epithelial cells shed, mild edema in C group, no obvious cell shedding. Conclusion Full abdominal irradiation can significantly damage the intestinal mucosa, causing bacterial translocation and endotoxemia, anti-E.coli IgY can significantly reduce the radiation damage to the small intestine mucosa.