单倍体马铃薯加倍的方法,一般是在二片子叶之间生长点上,放上用秋水仙素浸湿的棉花球,并用0.2～0.3％的秋水仙素水溶液,每日滴一次,每次滴几滴,连续五天。国外早在1955年列别杰娃,曾把种子放在用0.5％或0.7％秋水仙素浸湿过的滤纸上,使种子发芽,获得了加倍成功。 为了找到比较理想的加倍方法,使马铃薯单倍体的植株加倍成纯合的四倍体。我们利用已获得的马铃薯单倍体(2n=2x=24)植株为材料,采用两种方法进行了加倍试验。 Haploid potato doubling method, usually between the two cotyledons point of growth, put on cotton ball soaked with colchicine, and with 0.2 ~ 0.3% aqueous solution of colchicine drops once a day, each time Drop a few drops for five days. Foreign as early as 1955, Lebedeva, who had put seeds on the filter paper soaked with colchicine 0.5% or 0.7%, the seeds germinate, won the double success. In order to find the ideal method of doubling, potato haploid plants are doubled to homozygote tetraploids. We use the obtained potato haploid (2n = 2x = 24) plants as materials, using two methods to double test.