Joostella strains are emerging candidates for biosurfactant production.Here such ability was analyzed for Joostella strain A8 in comparison with Alcanivorax strain A53 and Pseudomonas strain A6,all previously isolated from hydrocarbon enrichment cultures made of polychaete homogenates.In pure cultures Joostella sp.A8 showed the highest stable emulsion percentage (78.33％),hydrophobicity rate (62.67％),and an optimal surface tension reduction during growth in mineral medium supplemented with diesel oil (reduction of about 12 mN/m),thus proving to be highly competitive with Alcanivorax and Pseudomonas strains.During growth in pure culture different level of biodegradation were detected for Alcanivorax strain A53 (52.7％),Pseudomonas strain A6 (38.2％) and Joostella strain A8 (26.8％).When growing in consortia,isolates achieved similar abundance values,with the best efficiency that was observed for the Joostella-Pseudonas co-culture.Gas-chromatographic analysis revealed an increase in the biodegradation efficiency in co-cultures (about 90％),suggesting that the contemporary action of different bacterial species could improve the process.Results were useful to compare the efficiencies of well-known biosurfactant producers (i.e.Pseudomonas and Alcanivorax representatives) with a still unknown biosuffactant producer,i.e.Joostella,and to confirm them as optimal biosurfactant-producing candidates.