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目的探讨不同染尘剂量大鼠矽肺模型血清中硅元素水平之间的剂量—反应关系并与可反映肺上皮细胞损伤和修复情况的细胞因子表面活性蛋白D(surface-active protein D,SP-D)进行比较。方法选取雄性Wistar大鼠按不同染尘剂量(10、30、50、70mg/ml)及不同时间点(染尘后1、3、5、7、14、21和28天)分为28个染尘组,取大鼠血液及肺组织,采用电感耦合等离子体质谱仪(ICP-MS)检测矽肺模型大鼠血清中硅元素含量,酶联免疫吸附测定法(ELISA法)定量测定大鼠血清中SP-D含量。结果随着染尘剂量的增加和时间的延长,模型组大鼠血清中硅元素的水平呈现逐渐增高的趋势;SP-D含量于造模后即开始增加,与对照组比较随染尘剂量及时间的增加呈逐渐增高然后下降的趋势。结论矽肺模型大鼠血清中硅元素水平在早期低剂量染尘时即明显升高,且早于SP-D的变化,二者水平均随染尘剂量和时间的不同而变化。提示,血清中硅元素和SP-D水平变化可能为矽肺的早期诊断提供生物学指标,具有一定的意义及价值。
Objective To investigate the dose-response relationship between serum silicon levels in different doses of dust-dosed silicosis model and to investigate the relationship between the dose-response and the level of surface-active protein D (SP-D), which can reflect the damage and repair of lung epithelial cells )Compare. Methods Male Wistar rats were divided into 28 dyed groups (10, 30, 50 and 70mg / ml) and different time points (1, 3, 5, 7, 14, 21 and 28 days after dying) In the dust group, the blood and lung tissues of the rats were taken out. The contents of silicon in the serum of the silicosis model rats were determined by inductively coupled plasma mass spectrometry (ICP-MS). The contents of silicon in the serum of the silicosis model rats were determined by enzyme-linked immunosorbent assay (ELISA) SP-D content. Results With the increase of the dose of dust and the prolongation of time, the level of silicon in the serum of model group showed a gradually increasing trend. The content of SP-D began to increase after the model was established. Compared with the control group, The increase of time showed a gradual increase and then decline. Conclusion The level of silicon in the serum of silicotic model rats significantly increased at the early low dose of dust exposure and earlier than the change of SP-D, both of them varied with the dose and time of dust exposure. Tip, changes in serum levels of silicon and SP-D silicosis may provide early diagnosis of biological indicators, has a certain significance and value.